Article
Genetic analysis of regions of the Lactococcus lactis subsp. lactis plasmid pRS01 involved in conjugative transfer.
Applied and environmental microbiology - 1 Dec 1994
Mills D A, Choi C K, Dunny G M, McKay L L
Abstract excerpt
The genes responsible for conjugative transfer of the 48.4-kb Lactococcus lactis subsp. lactis ML3 plasmid pRS01 were localized by insertional mutagenesis. Integration of the IS946-containing plasmid pTRK28 into pRS01 generated a pool of stable cointegrates, including a number of plasmids altered in conjugative proficiency. Mapping of pTRK28 insertions and phenotypic analysis of cointegrate plasmids identified...
Topics
- Chromosome Mapping
- Conjugation, Genetic
- DNA Transposable Elements
- Genes, Bacterial
- Genetic Complementation Test
- Lactococcus lactis
- Mutagenesis, Insertional
- Phenotype
- Plasmids