Article
Real-time genotyping of cytochrome P4501A1 A4889G and T6235C polymorphisms.
Molecular and cellular probes - 1 Apr 2001
Harth V, Brüning T, Abel J, Koch B, Berg I, Sachinidis A, Bolt H M, Vetter H, Ko Y
Abstract excerpt
Single nucleotide polymorphisms, such as the cytochrome P450 1A1 (CYP1A1) A4889G and T6235C variants, have been reported to be associated with an increased cancer risk. In order to study their role in molecular-epidemiological studies, we developed a single-step procedure for genotyping these two CYP1A1 polymorphisms using real-time polymerase chain reaction (PCR) and subsequent melting curve analysis. Genotypes...
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