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Article

A rapid and sensitive approach to mutation detection using real-time polymerase chain reaction and melting curve analyses, using BRCA1 as an example.

Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology - 1 Sept 1999

Pals G, Pindolia K, Worsham M J

Abstract excerpt

BACKGROUND: The detection of 2 recurrent mutations in the BRCA1 gene (Ashkenazi Jewish and Dutch populations) was studied with real-time polymerase chain reaction (PCR) and melting curve analyses. METHODS: PCR products were designed around the 185delAG in exon 2 and the single- base substitution 2841G>T in exon 11. Hybridization probe sets were designed for both PCR products, with each probe overlapping the...

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Share to DNA, Neoplasm
Share to Genes, BRCA1

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Heteroduplex Analysis

Share to Mutagenesis, Insertional

Share to Neoplastic Syndromes, Hereditary

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Share to Nucleic Acid Denaturation

Source

Identifiers

PMID: 10553024
DOI: 10.1016/s1084-8592(99)80027-7

Genes

BRCA1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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