Structure-function studies of an IGF-I analogue that can be chemically cleaved to a two-chain mini-IGF-I.

doi:10.1093/protein/14.1.61

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Article

Structure-function studies of an IGF-I analogue that can be chemically cleaved to a two-chain mini-IGF-I.

Protein engineering - 1 Jan 2001

Geddes S, Holst P, Grotzinger J, Gill R, Nugent P, De Meyts P, Wollmer A, Wood S, Pitts J

Abstract excerpt

The structure and biological activities of two disulphide isomers of a C-region deletion mutant of insulin-like growth factor-I (IGF-I) which has an Asn--Gly link engineered at the junction of the A- and B-regions were studied before and after chemical cleavage. Circular dichroism (CD) spectra and binding affinity to IGF binding protein 3 (IGFBP3) indicated that the treatment with hydroxylamine did not disrupt...

Topics

3T3 Cells
Adipocytes
Amino Acid Sequence
Animals
Binding Sites
Carrier Proteins
Cell Line
Circular Dichroism
Insulin-Like Growth Factor I
Insulin-Like Growth Factor II
Mice

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutagenesis, Site-Directed

Mutation

Peptides

Protein Conformation

Protein Structure, Tertiary

Rats

Receptor, IGF Type 1

Source

Open in PubMed

Identifiers

PMID: 11287679
DOI: 10.1093/protein/14.1.61

Genes

IGFBP3

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

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