Article
Structural changes in insulin-like growth factor (IGF) I mutant proteins affecting binding kinetic rates to IGF binding protein 1 and IGF-I receptor.
Biochemistry - 8 Apr 1997
Jansson M, Uhlen M, Nilsson B
Abstract excerpt
Ligand binding properties of five single amino acid substituted variants (V11A, D12A, Q15A, Q15E, and F16A) of human insulin-like growth factor I (IGF-I) were analyzed with respect to their binding affinities and binding kinetics to recombinant IGF binding protein 1 (IGFBP-1) and a soluble form o...
Topics
- Amino Acid Sequence
- Binding, Competitive
- Biosensing Techniques
- Circular Dichroism
- DNA Primers
- Humans
- Insulin-Like Growth Factor Binding Protein 1
- Insulin-Like Growth Factor I
- Kinetics
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Pepsin A
- Peptide Fragments
- Protein Binding
- Protein Structure, Secondary
- Receptor, IGF Type 1