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Article

Structural mechanism of ribonucleotide discrimination by a Y-family DNA polymerase.

Journal of molecular biology - 1 Apr 2011

Kirouac Kevin N, Suo Zucai, Ling Hong

Abstract excerpt

The ability of DNA polymerases to differentiate between ribonucleotides and deoxribonucleotides is fundamental to the accurate replication and maintenance of an organism's genome. The active sites of Y-family DNA polymerases are highly solvent accessible, yet these enzymes still maintain a high selectivity towards deoxyribonucleotides. Here, we biochemically demonstrate that a single active-site mutation (Y12A)...

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Identifiers

PMID: 21295588
DOI: 10.1016/j.jmb.2011.01.037

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