Article
[The study of the metal-binding region in human growth hormone using immobilized metal ion affinity gel-electrophoresis].
Bioorganicheskaia khimiia - 1 Jan 2000
Anisimova M V, Shul'ga A A, Levichkin I V, Kirpichnikov M P, Poliakov K M, Skriabin K G, Vijayalakshmi M A, Varlamov V P
Abstract excerpt
The zinc(II)-binding affinities of recombinant human growth hormone and two its mutants, 14-33 and 14-95, were studied using Immobilized Metal Ion Affinity Gel-electrophoresis (IMAG). The mutant hormones, composed of polypeptide chain segments of the human and porcine growth hormones, lacked His18, which may be crucial for binding of the intact hormone to the transition metal ions. The mutations did not affect...
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