Article
[A universal method of purifying genetically engineered human somatotropin and its mutants using metallochelating agent affinity chromatography].
Biokhimiia (Moscow, Russia) - 1 Feb 1994
Lopatin S A, Il'ina A V, Shul'ga A A, Grinchenko O S, Varlamov V P, Skriabin K G, Kirpichnikov M P
Abstract excerpt
A novel scheme for human recombinant somatotropin and its mutant forms isolation which includes ion-exchange and metal chelate affinity chromatography has been developed. A change in the chromatographic behaviour of the isolated proteins depending on the amino acid substitutions has been demonstrated.
Topics
- Chelating Agents
- Chromatography, Affinity
- Chromatography, Ion Exchange
- Electrophoresis, Polyacrylamide Gel
- Growth Hormone
- Humans
- Metals
- Mutation
- Recombinant Proteins