Article
Important role of the tetraloop region of 4.5S RNA in SRP binding to its receptor FtsY.
RNA (New York, N.Y.) - 1 Feb 2001
Jagath J R, Matassova N B, de Leeuw E, Warnecke J M, Lentzen G, Rodnina M V, Luirink J, Wintermeyer W
Abstract excerpt
Binding of Escherichia coli signal recognition particle (SRP) to its receptor, FtsY, requires the presence of 4.5S RNA, although FtsY alone does not interact with 4.5S RNA. In this study, we report that the exchange of the GGAA tetraloop sequence in domain IV of 4.5S RNA for UUCG abolishes SRP-FtsY interaction, as determined by gel retardation and membrane targeting experiments, whereas replacements with other...
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