Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Determination of the mutation rate of poliovirus RNA-dependent RNA polymerase.

Virus research - 1 Apr 2001

Wells V R, Plotch S J, DeStefano J J

Abstract excerpt

The fidelity of poliovirus RNA-dependent RNA polymerase (3D(pol)) was determined using a system based on the fidelity of synthesis of the alpha-lac gene which codes for a subunit of beta-galactosidase. Synthesis products are screened for mutations by an alpha-complementation assay, in which the protein product from alpha-lac is used in trans to complement beta-galactosidase activity in bacteria that do not...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to beta-Galactosidase
Share to DNA, Recombinant
Share to Escherichia coli

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Genetic Vectors

Share to Poliovirus

Share to RNA-Dependent RNA Polymerase

Share to Substrate Specificity

Share to Templates, Genetic

Source

Identifiers

PMID: 11226580
DOI: 10.1016/s0168-1702(00)00256-2

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Determination of the mutation rate of poliovirus RNA-dependent RNA polymerase.PMID 11226580Read ↗

Fidelity and error specificity of the alpha catalytic subunit of Escherichia coli DNA polymerase III.Semantic proximity Read ↗

A plasmid-based lacZα gene assay for DNA polymerase fidelity measurement.Semantic proximity Read ↗

Fidelity of HIV-1 reverse transcriptase copying RNA in vitro.Semantic proximity Read ↗

Isolation of fidelity variants of RNA viruses and characterization of virus mutation frequency.Semantic proximity Read ↗

High Fidelity of Yellow Fever Virus RNA PolymeraseSemantic proximity Read ↗

Determinants of RNA-dependent RNA polymerase (in)fidelity revealed by kinetic analysis of the polymerase encoded by a foot-and-mouth disease virus mutant with reduced sensitivity to ribavirin.Semantic proximity Read ↗

Structure-function relationships of the viral RNA-dependent RNA polymerase: fidelity, replication speed, and initiation mechanism determined by a residue in the ribose-binding pocket.Semantic proximity Read ↗

Enzymatic activity of poliovirus RNA polymerases with mutations at the tyrosine residue of the conserved YGDD motif: isolation and characterization of polioviruses containing RNA polymerases with FGDD and MGDD sequences.Semantic proximity Read ↗

High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase.Semantic proximity Read ↗

Foot-and-mouth disease virus low-fidelity polymerase mutants are attenuated.Semantic proximity Read ↗

Biochemical characterization of the fidelity of poliovirus RNA-dependent RNA polymerase.Semantic proximity Read ↗

Escherichia coli DNA polymerase III epsilon subunit increases Moloney murine leukemia virus reverse transcriptase fidelity and accuracy of RT-PCR procedures.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.