Article
Analysis of C-MYC function in normal cells via conditional gene-targeted mutation.
Immunity - 1 Jan 2001
de Alboran I M, O'Hagan R C, Gärtner F, Malynn B, Davidson L, Rickert R, Rajewsky K, DePinho R A, Alt F W
Abstract excerpt
Germline inactivation of c-myc in mice causes embryonic lethality. Therefore, we developed a LoxP/Cre-based conditional mutation approach to test the role of c-myc in mouse embryonic fibroblasts (MEFs) and mature B lymphocytes. Cre expression resulted in reduced proliferation of wild-type MEFs, but c-Myc-deficient MEFs showed a further reduction. In contrast to fibroblasts, Cre expression had no apparent affect...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.