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Article

Directed evolution of operon of trehalose-6-phosphate synthase/phosphatase from Escherichia coli.

Biochemical and biophysical research communications - 12 Jan 2001

Kong X, Liu Y, Gou X, Zhang H, Wang X, Zhang J

Abstract excerpt

Trehalose is a nonspecific protective agent for biomacromolecules. Trehalose-6-phosphate synthase (OtsA)/phosphatase (OtsB), which is encoded by the gene operon otsBA located at -42 of the Escherichia coli genome, is the main enzyme system that catalyzes the synthesis of trehalose in E. coli. We cloned the operon and modified it by directed evolution. Unlike in the previously reported work, we modified the whole...

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PMID: 11162529
DOI: 10.1006/bbrc.2000.3819

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Source receipt: pubmed, retrieved 7 Sept 2026.

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