Article
Cloning and truncation modification of trehalose-6-phosphate synthase gene from Selaginella pulvinata.
Gene - 10 Jan 2013
Zhao Sheng-Mei, Fu Feng-Ling, Gou Lin, Wang Han-Guang, He Gang, Li Wan-Chen
Abstract excerpt
A homologous sequence was amplified from resurrection plant Selaginella pulvinta by RACE technique, proved to be the full-length cDNA of trehalose-6-phosphate synthase gene by homologous alignment and yeast complementation assay, and nominated as SpTPS1 gene. The open reading frame of this gene was truncated 225bp at the 5'-end, resulting the N-terminal truncation modification of 75 amino acids for its encoding...
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