Article
Effects of mutations in the calcium-binding sites of recoverin on its calcium affinity: evidence for successive filling of the calcium binding sites.
Protein engineering - 1 Nov 2000
Permyakov S E, Cherskaya A M, Senin I I, Zargarov A A, Shulga-Morskoy S V, Alekseev A M, Zinchenko D V, Lipkin V M, Philippov P P, Uversky V N, Permyakov E A
Abstract excerpt
A molecule of the photoreceptor Ca(2+)-binding protein recoverin contains four potential EF-hand Ca(2+)-binding sites, of which only two, the second and the third, are capable of binding calcium ions. We have studied the effects of substitutions in the second, third and fourth EF-hand sites of recoverin on its Ca(2+)-binding properties and some other characteristics, using intrinsic fluorescence, circular...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.