Article
Obtaining and characterization of EF-hand mutants of recoverin.
FEBS letters - 27 Nov 1998
Alekseev A M, Shulga-Morskoy S V, Zinchenko D V, Shulga-Morskaya S A, Suchkov D V, Vaganova S A, Senin I I, Zargarov A A, Lipkin V M, Akhtar M, Philippov P P
Abstract excerpt
Several EF-hand recoverin mutants were obtained and their abilities to bind to photoreceptor membranes and to inhibit rhodopsin kinase were determined. The mutants with the 'spoiled' 2nd, 3rd or (2nd+3rd) EF-hand structures did not act upon the kinase activity in the microM range of Ca2+ concentr...
Topics
- Animals
- Binding Sites
- Calcium
- Calcium-Binding Proteins
- Cattle
- Eye Proteins
- G-Protein-Coupled Receptor Kinase 1
- Hippocalcin
- Lipoproteins
- Mutagenesis, Site-Directed
- Mutation
- Nerve Tissue Proteins
- Phenotype
- Phosphorylation
- Protein Kinase Inhibitors