Article
Obtaining and characterization of EF-hand mutants of recoverin.
FEBS letters - 27 Nov 1998
Alekseev A M, Shulga-Morskoy S V, Zinchenko D V, Shulga-Morskaya S A, Suchkov D V, Vaganova S A, Senin I I, Zargarov A A, Lipkin V M, Akhtar M, Philippov P P
Abstract excerpt
Several EF-hand recoverin mutants were obtained and their abilities to bind to photoreceptor membranes and to inhibit rhodopsin kinase were determined. The mutants with the 'spoiled' 2nd, 3rd or (2nd+3rd) EF-hand structures did not act upon the kinase activity in the microM range of Ca2+ concentrations. Mutations of the 4th EF hand, which 'repaired' its Ca2+-binding activity, resulted in recoverin with three...
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