Article
Differential termination of primer extension: a novel, quantifiable method for detection of point mutations.
Human genetics - 1 May 1992
Picketts D J, Cameron C, Taylor S A, Deugau K V, Lillicrap D P
Abstract excerpt
We have developed a novel technique that is useful for the rapid detection of previously characterized point mutations in the human genome. The method relies on the differential termination of primer extension being performed simultaneously on normal and mutant template molecules in the presence...
Topics
- Base Sequence
- Factor IX
- Female
- Genetic Carrier Screening
- Hemophilia B
- Humans
- Male
- Molecular Sequence Data
- Mutation
- Oligodeoxyribonucleotides
- Polymerase Chain Reaction