Article
An infrared fluorescent dATP for labeling DNA.
Genome research - 1 Nov 1995
Steffens D L, Jang G Y, Sutter S L, Brumbaugh J A, Middendorf L R, Mühlegger K, Mardis E R, Weinstock L A, Wilson R K
Abstract excerpt
Near-infrared fluorescence provides a nonradioactive method of detection with high sensitivity and low background. An infrared fluorophore has been attached covalently to the nucleotide deoxyadenosine triphosphate (dATP) to provide a reagent for enzymatic labeling of various types of DNA molecules and for facilitating their detection with an automated DNA sequencing and analysis system. DNA sequencing reaction...
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