Article
Evaluation of PCR-generated chimeras, mutations, and heteroduplexes with 16S rRNA gene-based cloning.
Applied and environmental microbiology - 1 Feb 2001
Qiu X, Wu L, Huang H, McDonel P E, Palumbo A V, Tiedje J M, Zhou J
Abstract excerpt
To evaluate PCR-generated artifacts (i.e., chimeras, mutations, and heteroduplexes) with the 16S ribosomal DNA (rDNA)-based cloning approach, a model community of four species was constructed from alpha, beta, and gamma subdivisions of the division Proteobacteria as well as gram-positive bacteriu...
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