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Article

Sample richness and genetic diversity as drivers of chimera formation in nSSU metagenetic analyses.

Nucleic acids research - 1 May 2012

Fonseca V G, Nichols B, Lallias D, Quince C, Carvalho G R, Power D M, Creer S

Abstract excerpt

Eukaryotic diversity in environmental samples is often assessed via PCR-based amplification of nSSU genes. However, estimates of diversity derived from pyrosequencing environmental data sets are often inflated, mainly because of the formation of chimeric sequences during PCR amplification. Chimeras are hybrid products composed of distinct parental sequences that can lead to the misinterpretation of diversity...

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PMID: 22278883
DOI: 10.1093/nar/gks002
PMCID: PMC3351157

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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