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Measuring rDNA diversity in eukaryotic microbial systems: how intragenomic variation, pseudogenes, and PCR artifacts confound biodiversity estimates.

Molecular ecology - 1 Dec 2007

Thornhill Daniel J, Lajeunesse Todd C, Santos Scott R

Abstract excerpt

Molecular approaches have revolutionized our ability to study the ecology and evolution of micro-organisms. Among the most widely used genetic markers for these studies are genes and spacers of the rDNA operon. However, the presence of intragenomic rDNA variation, especially among eukaryotes, can potentially confound estimates of microbial diversity. To test this hypothesis, bacterially cloned PCR products of the...

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PMID: 17995924
DOI: 10.1111/j.1365-294X.2007.03576.x

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