Article
Microvariation artifacts introduced by PCR and cloning of closely related 16S rRNA gene sequences.
Applied and environmental microbiology - 1 Jan 2001
Speksnijder A G, Kowalchuk G A, De Jong S, Kline E, Stephen J R, Laanbroek H J
Abstract excerpt
A defined template mixture of seven closely related 16S-rDNA clones was used in a PCR-cloning experiment to assess and track sources of artifactual sequence variation in 16S rDNA clone libraries. At least 14% of the recovered clones contained aberrations. Artifact sources were polymerase errors, a mutational hot spot, and cloning of heteroduplexes and chimeras. These data may partially explain the high degree of...
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