Article
Strategy and implementation of a system for protein engineering.
Journal of biotechnology - 1 Mar 1993
Goddette D W, Christianson T, Ladin B F, Lau M, Mielenz J R, Paech C, Reynolds R B, Yang S S, Wilson C R
Abstract excerpt
This paper describes an overall view of an industrial protein engineering project from conception to successful completion. The choice of rational design was determined by the availability of an excellent three-dimensional crystal structure and the availability of information in the literature to define a strategy. The design strategy was refined extensively during the course of the project. The development of...
Topics
- Bacillus
- Chromatography, High Pressure Liquid
- Detergents
- Enzyme Stability
- Industrial Microbiology
- Kinetics
- Mutagenesis, Site-Directed
- Mutation
- Protein Engineering
- Serine Endopeptidases
- Subtilisins