Article
Development and characterization of a xylose-dependent system for expression of cloned genes in Bacillus subtilis: conditional complementation of a teichoic acid mutant.
Applied and environmental microbiology - 1 Jan 2001
Bhavsar A P, Zhao X, Brown E D
Abstract excerpt
We have developed a xylose-dependent expression system for tight and modulated expression of cloned genes in Bacillus subtilis. The expression system is contained on plasmid pSWEET for integration at the amyE locus of B. subtilis and incorporates components of the well-characterized, divergently transcribed xylose utilization operon. The system contains the xylose repressor encoded by xylR, the promoter and 5'...
Topics
- Bacillus subtilis
- Cloning, Molecular
- DNA Primers
- Gene Expression
- Genetic Complementation Test
- Mutation
- Nucleotidyltransferases
- Operon
- Plasmids
- Teichoic Acids
- Xylose
- beta-Galactosidase