Article
An operator binding-negative mutation of Xyl repressor from Bacillus subtilis is trans dominant in Bacillus megaterium.
FEMS microbiology letters - 1 May 1993
Kauder C, Allmansberger R, Gärtner D, Schmiedel D, Hillen W
Abstract excerpt
We have selected a Bacillus subtilis 168-borne xylR Ser to Leu mutation at position 41 of the encoded amino acid sequence showing a constitutive expression phenotype for the xyl operon. When cloned on a multi-copy plasmid in a B. megaterium strain harbouring a single-copy xylA-lacZ fusion it leads to derepression of beta-galactosidase expression. Thus, it is trans dominant over the endogenous xylR, indicating...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.