Article
Role of excess inorganic pyrophosphate in primer-extension genotyping assays.
Genome research - 1 Sept 2004
Xiao Ming, Phong Angie, Lum Kristen L, Greene Richard A, Buzby Philip R, Kwok Pui-Yan
Abstract excerpt
We have developed and genotyped >15,000 SNP assays by using a primer extension genotyping assay with fluorescence polarization (FP) detection. Although the 80% success rate of this assay is similar to those of other SNP genotyping assays, we wanted to determine the reasons for the failures and fi...
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