Article
Template tailoring: Accurate determination of heterozygous alleles using peptide nucleic acid and dideoxyNTP.
Electrophoresis - 1 Apr 2010
Tariq Muhammad Akram, Pourmand Nader
Abstract excerpt
Measurement of the length of DNA fragments plays a pivotal role in genetic mapping, disease diagnostics, human identification and forensic applications. PCR followed by electrophoresis is used for DNA length measurement of STRs, a process that requires labeled primers and allelic ladders as standards to avoid machine error. Sequencing-based approaches can be used for STR analysis to eliminate the requirement of...
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