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Testing the 3Q:1R “Rule”: Mutational Analysis of the Ionic “Zero” Layer in the Yeast Exocytic SNARE Complex Reveals No Requirement for Arginine

1 Nov 2000

Abstract excerpt

The crystal structure of the synaptic SNARE complex reveals a parallel four-helix coiled-coil arrangement; buried in the hydrophobic core of the complex is an unusual ionic layer composed of three glutamines and one arginine, each provided by a separate alpha-helix. The presence of glutamine or arginine residues in this position is highly conserved across the t- and v-SNARE families, and it was recently suggested...

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PMID: 11071911
DOI: 10.1091/mbc.11.11.3849

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Related research

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Testing the 3Q:1R “Rule”: Mutational Analysis of the Ionic “Zero” Layer in the Yeast Exocytic SNARE Complex Reveals No Requirement for ArgininePMID 11071911Read ↗

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Use of G-protein fusions to monitor integral membrane protein-protein interactions in yeast.Semantic proximity Read ↗

Structural transitions in the synaptic SNARE complex during Ca2+-triggered exocytosis.Semantic proximity Read ↗

Conserved alpha-helical segments on yeast homologs of the synaptobrevin/VAMP family of v-SNAREs mediate exocytic function.Semantic proximity Read ↗

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