Article
Use of G-protein fusions to monitor integral membrane protein-protein interactions in yeast.
Nature biotechnology - 1 Oct 2000
Ehrhard K N, Jacoby J J, Fu X Y, Jahn R, Dohlman H G
Abstract excerpt
The control of protein-protein interactions is a fundamental aspect of cell regulation. Here we describe a new approach to detect the interaction of two proteins in vivo. By this method, one binding partner is an integral membrane protein whereas the other is soluble but fused to a G-protein gamma-subunit. If the binding partners interact, G-protein signaling is disrupted. We demonstrate interaction between known...
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