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Article

A random mutagenesis approach to isolate dominant-negative yeast sec1 mutants reveals a functional role for domain 3a in yeast and mammalian Sec1/Munc18 proteins.

Genetics - 1 Sept 2008

Boyd Alan, Ciufo Leonora F, Barclay Jeff W, Graham Margaret E, Haynes Lee P, Doherty Mary K, Riesen Michèle, Burgoyne Robert D, Morgan Alan

Abstract excerpt

SNAP receptor (SNARE) and Sec1/Munc18 (SM) proteins are required for all intracellular membrane fusion events. SNAREs are widely believed to drive the fusion process, but the function of SM proteins remains unclear. To shed light on this, we screened for dominant-negative mutants of yeast Sec1 by random mutagenesis of a GAL1-regulated SEC1 plasmid. Mutants were identified on the basis of galactose-inducible...

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Identifiers

PMID: 18757920
DOI: 10.1534/genetics.108.090423
PMCID: PMC2535671

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Source receipt: pubmed, retrieved 7 Sept 2026.

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