Article
Geranylgeranylated SNAREs are dominant inhibitors of membrane fusion.
The Journal of cell biology - 16 Oct 2000
Grote E, Baba M, Ohsumi Y, Novick P J
Abstract excerpt
Exocytosis in yeast requires the assembly of the secretory vesicle soluble N-ethylmaleimide-sensitive factor attachment protein receptor (v-SNARE) Sncp and the plasma membrane t-SNAREs Ssop and Sec9p into a SNARE complex. High-level expression of mutant Snc1 or Sso2 proteins that have a COOH-terminal geranylgeranylation signal instead of a transmembrane domain inhibits exocytosis at a stage after vesicle docking....
Topics
- Cell Membrane
- Diterpenes
- Exocytosis
- Fungal Proteins
- Lipoproteins
- Membrane Fusion
- Membrane Proteins
- Models, Biological
- Mutation
- Protein Prenylation
- Protein Structure, Tertiary