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Article

Rapid small-scale isolation of SV40 virions and SV40 DNA.

Journal of virological methods - 1 Nov 2000

Orlando S J, Nabavi M, Gharakhanian E

Abstract excerpt

A rapid method for the small-scale isolation of SV40 virions and SV40 DNA is presented. CV-1 monkey epithelial cells are transfected with linear SV40 DNA. After the onset of transfection, cells are lysed by several freeze/thaw cycles and virions are isolated using polyethylene glycol (PEG) precipitation of DNase I treated lysates. Viral DNA is released by proteinase K and dithiothreitol treatment of the isolated...

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PMID: 11064111
DOI: 10.1016/s0166-0934(00)00176-2

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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