Article
Identification of the functional site in the mosquito larvicidal binary toxin of Bacillus sphaericus 1593M by site-directed mutagenesis.
Biochemical and biophysical research communications - 5 Oct 2000
Elangovan G, Shanmugavelu M, Rajamohan F, Dean D H, Jayaraman K
Abstract excerpt
To study the mode of action of the binary toxin (51- and 42-kDa) of Bacillus sphaericus, amino acid residues were substituted at selected sites of the N- and C-terminal regions of both peptides. Bioassay results of the mutant binary toxins tested against mosquito larvae, Culex quinquefasciatus, revealed that most of the substitutions made on both peptides led to either decrease or total loss of the activity....
Topics
- Animals
- Bacillus
- Bacterial Toxins
- Binding, Competitive
- Biological Assay
- Culex
- Cytoplasmic Vesicles
- Genetic Complementation Test
- Larva
- Microvilli
- Molecular Weight
- Mutagenesis, Site-Directed
- Mutation
- Peptide Fragments
- Protein Binding
- Thermodynamics