Article
Directed mutagenesis of selected regions of a Bacillus thuringiensis entomocidal protein.
FEMS microbiology letters - 1 Mar 1990
Ahmad W, Ellar D J
Abstract excerpt
Comparison of the sequences of Bacillus thuringiensis entomocidal toxins of widely differing specificity reveals six conserved domains. The role of one of the most highly conserved domains (D1) located near the N-terminus has been investigated by site directed mutagenesis at two positions. Althou...
Topics
- Animals
- Bacillus thuringiensis
- Bacterial Toxins
- Base Sequence
- Electrophoresis, Polyacrylamide Gel
- Immunoblotting
- Insecta
- Insecticides
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Protein Precursors
- Recombinant Proteins
- Sequence Homology, Nucleic Acid
- Species Specificity