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Article

Construction by site-directed mutagenesis of a 39-kilodalton mosquitocidal protein similar to the larva-processed toxin of Bacillus sphaericus 2362.

Journal of bacteriology - 1 Jul 1990

Broadwell A H, Clark M A, Baumann L, Baumann P

Abstract excerpt

After ingestion of the parasporal crystals of Bacillus sphaericus, mosquito larvae process the 42-kilodalton (kDa) toxin to a protein of 39 kDa, which has an increased toxicity (A. H. Broadwell and P. Baumann, Appl. Environ. Microbiol. 53:1333-1337, 1987). A similar activation is performed by trypsin and chymotrypsin. Using site-directed mutagenesis, we have constructed derivatives of the 42-kDa toxin with a...

Topics

Amino Acid Sequence
Animals
Bacillus
Bacillus subtilis
Bacterial Proteins
Bacterial Toxins
Base Sequence
Culex
Gene Expression
Larva
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Weight

Mutation

Oligonucleotide Probes

Restriction Mapping

Source

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Identifiers

PMID: 2113918
DOI: 10.1128/jb.172.7.4032-4036.1990
PMCID: PMC213389

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2113918-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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