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Article

Functional complementation of nontoxic mutant binary toxins of Bacillus sphaericus 1593M generated by site-directed mutagenesis.

Applied and environmental microbiology - 1 Feb 1998

Shanmugavelu M, Rajamohan F, Kathirvel M, Elangovan G, Dean D H, Jayaraman K

Abstract excerpt

Alanine residues were substituted by site-directed mutagenesis at selected sites of the N- and C-terminal regions of the binary toxin (51- and 42-kDa peptides) of B. sphaericus 1593M, and the mutant toxins were cloned and expressed in Escherichia coli. Bioassays with mosquito larvae, using binary toxins derived from individual mutants, showed that the substitution of alanine at some sites in both the 51-kDa and...

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Identifiers

PMID: 9464418
DOI: 10.1128/AEM.64.2.756-759.1998
PMCID: PMC106113

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