Article
Targeted mutagenesis at charged residues in Bacillus sphaericus BinA toxin affects mosquito-larvicidal activity.
Current microbiology - 1 Sept 2008
Sanitt Poohrawind, Promdonkoy Boonhiang, Boonserm Panadda
Abstract excerpt
The mosquito-larvicidal binary toxin from Bacillus sphaericus is composed of two polypeptides called BinA and BinB with molecular masses of approximately 42 and 51 kDa. Both components are required for full activity, with BinB acting as a specificity determinant and BinA being responsible for toxic action. To investigate the role of the selected charged residues in BinA, four mutants were generated by replacing...
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