Article
Real-time quantitative polymerase chain reaction in cardiac transplant research.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2006
Felkin Leanne E, Taegtmeyer Anne B, Barton Paul J R
Abstract excerpt
The real-time quantitative polymerase chain reaction (PCR), an increasingly popular technique for the detection of DNA, combines a high degree of accuracy with extreme sensitivity. In this chapter we describe the use of real-time quantitative PCR in transplantation research in two areas in which this method is commonly applied: the accurate quantification of mRNA in tissue samples and genotyping of DNA. These are...
Topics
- Animals
- DNA
- Heart Transplantation
- Humans
- Polymorphism, Single Nucleotide
- RNA, Messenger
- Research
- Reverse Transcriptase Polymerase Chain Reaction
