Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Validation of optimal reference genes for quantitative real time PCR in muscle and adipose tissue for obesity and diabetes research.

Scientific reports - 15 Jun 2017

Perez Lester J, Rios Liliam, Trivedi Purvi, D'Souza Kenneth, Cowie Andrew, Nzirorera Carine, Webster Duncan, Brunt Keith, Legare Jean-Francois, Hassan Ansar, Kienesberger Petra C, Pulinilkunnil Thomas

Abstract excerpt

The global incidence of obesity has led to an increasing need for understanding the molecular mechanisms that drive this epidemic and its comorbidities. Quantitative real-time RT-PCR (RT-qPCR) is the most reliable and widely used method for gene expression analysis. The selection of suitable reference genes (RGs) is critical for obtaining accurate gene expression information. The current study aimed to identify...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Adipose Tissue
Share to Diabetes Mellitus

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Genetic Association Studies

Share to Muscles

Share to Myoblasts

Share to Obesity

Share to Real-Time Polymerase Chain Reaction

Share to Reproducibility of Results

Source

Open in PubMed Open full text

Identifiers

PMID: 28620170
DOI: 10.1038/s41598-017-03730-9
PMCID: PMC5472619

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Validation of optimal reference genes for quantitative real time PCR in muscle and adipose tissue for obesity and diabetes research.PMID 28620170Read ↗

Evaluation of candidate reference genes for RT-qPCR studies in three metabolism related tissues of mice after caloric restrictionSemantic proximity Read ↗

Validation of endogenous reference genes for qRT-PCR analysis of human visceral adipose samplesSemantic proximity Read ↗

Validation of Reference Genes for the Relative Quantification of Gene Expression in Human Epicardial Adipose TissueSemantic proximity Read ↗

Tissue-specific selection of stable reference genes for real-time PCR normalization in an obese rat modelSemantic proximity Read ↗

Impact of reference gene selection for target gene normalization on experimental outcome using real-time qRT-PCR in adipocytes.Semantic proximity Read ↗

Evaluation of the stability of standard reference genes of adipose-derived mesenchymal stem cells during in vitro proliferation and differentiation.Semantic proximity Read ↗

Evidence based selection of commonly used RT-qPCR reference genes for the analysis of mouse skeletal muscle.Semantic proximity Read ↗

Recommendations for improving accuracy of gene expression data in bone and cartilage tissue engineeringSemantic proximity Read ↗

Identification of stable reference genes for quantitative real-time PCR in human fibroblasts from lymph nodes and synoviumSemantic proximity Read ↗

Novel porcine housekeeping genes for real-time RT-PCR experiments normalization in adipose tissue: assessment of leptin mRNA quantity in different pig breeds.Semantic proximity Read ↗

A functional genomic approach to identify reference genes for human pancreatic beta cell real-time quantitative RT-PCR analysisSemantic proximity Read ↗

Reference genes in real-time PCR.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.