Article
Error-prone PCR of Vitreoscilla hemoglobin (VHb) to support the growth of microaerobic Escherichia coli.
Biotechnology and bioengineering - 20 Nov 2000
Andersson C I, Holmberg N, Farrés J, Bailey J E, Bülow L, Kallio P T
Abstract excerpt
Expression of the gene encoding bacterial hemoglobin (VHb) from Vitreoscilla has been previously used to improve recombinant cell growth and enhance product formation under microaerobic conditions. It is very likely that the properties of VHb are not optimized for foreign hosts; therefore, we used error-prone PCR to generate a number of randomly mutated vhb genes to be expressed and studied in Escherichia coli....
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