Article
Improved acid tolerance of Lactobacillus pentosus by error-prone whole genome amplification.
Bioresource technology - 1 May 2013
Ye Lidan, Zhao Hua, Li Zhi, Wu Jin Chuan
Abstract excerpt
Acid tolerance of Lactobacillus pentosus ATCC 8041 was improved by error-prone amplification of its genomic DNA using random primers and Taq DNA polymerase. The resulting amplification products were transferred into wild-type L. pentosus by electroporation and the transformants were screened for growth on low-pH agar plates. After only one round of mutation, one mutant (MT3) was identified that was able to...
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