Article
Enhanced ribosome and tRNA contents in Escherichia coli expressing a truncated Vitreoscilla hemoglobin mutant analyzed by flow field-flow fractionation.
Biotechnology letters - 1 Sept 2003
Andersson Charlotte I J, Arfvidsson Cecilia, Kallio Pauli T, Wahlund Karl-Gustav, Bülow Leif
Abstract excerpt
The ribosome and tRNA levels of Escherichia coli cells, transformed with a native or mutated Vitreoscilla hemoglobin genes (vhb), were investigated using asymmetrical flow field-flow fractionation (AFFFF). Mutagenesis of rhb by error-prone PCR was carried out to alter the growth behavior of microaerobically cultivated native VHb-expressing E. coli. A VHb mutant, pVMT1, was identified, which was able to reach a...
Topics
- Bacterial Proteins
- Cell Division
- Escherichia coli
- Fractionation, Field Flow
- Genetic Enhancement
- Hemoglobins
- Mutation
- Protein Engineering
- RNA, Transfer
- Recombinant Proteins