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Article

A mutation that improves soluble recombinant hemoglobin accumulation in Escherichia coli in heme excess.

Applied and environmental microbiology - 1 Feb 1999

Weickert M J, Pagratis M, Glascock C B, Blackmore R

Abstract excerpt

High-level expression of soluble recombinant human hemoglobin (rHb) in Escherichia coli was obtained with several hemoglobin variants. Under identical conditions, two rHbs containing the Presbyterian mutation (Asn-108-->Lys) in beta-globin accumulated to approximately twofold less soluble globin than rHbs containing the corresponding wild-type beta-globin subunit accumulated. The beta-globin Providence(asp)...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 9925594
DOI: 10.1128/AEM.65.2.640-647.1999
PMCID: PMC91073

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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