Cloning and nucleotide sequence of the DNA gyrase (gyrA) gene from Mycoplasma hominis and characterization of quinolone-resistant mutants selected in vitro with trovafloxacin.

doi:10.1128/AAC.44.10.2719-2727.2000

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Article

Cloning and nucleotide sequence of the DNA gyrase (gyrA) gene from Mycoplasma hominis and characterization of quinolone-resistant mutants selected in vitro with trovafloxacin.

Antimicrobial agents and chemotherapy - 1 Oct 2000

Bébéar C M, Grau O, Charron A, Renaudin H, Gruson D, Bébéar C

Abstract excerpt

We report the cloning and characterization of the gyrA gene of the Mycoplasma hominis DNA gyrase, which was previously shown to be associated with quinolone resistance in this organism. The 2,733-bp gyrA gene encodes a protein of 911 amino acids with a calculated molecular mass of 102.5 kDa. As expected, M. hominis GyrA exhibits higher homology with the GyrA subunits of the gram-positive bacteria Clostridium...

Topics

Amino Acid Sequence
Anti-Infective Agents
Bacterial Proteins
Base Sequence
Cloning, Molecular
DNA Gyrase
DNA Topoisomerase IV
DNA Topoisomerases, Type II
DNA, Bacterial
DNA-Binding Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Drug Resistance, Microbial

Electrophoresis, Gel, Pulsed-Field

Fluoroquinolones

Molecular Sequence Data

Mutation

Mycoplasma hominis

Naphthyridines

Plasmids

Source

Open in PubMed Open full text

Identifiers

PMID: 10991851
DOI: 10.1128/AAC.44.10.2719-2727.2000
PMCID: PMC90142

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-10991851-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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