Article
Characterization of Mycoplasma hominis mutations involved in resistance to fluoroquinolones.
Antimicrobial agents and chemotherapy - 1 Feb 1997
Bebear C M, Bové J M, Bebear C, Renaudin J
Abstract excerpt
Fluoroquinolone-resistant mutants of Mycoplasma hominis were selected in vitro from the PG21 susceptible reference strain either by multistep selection on increasing concentrations of various fluoroquinolones or by one-step selection on agar medium with ofloxacin. The quinolone resistance-determining regions (QRDR) of the structural genes encoding the A and b subunits of DNA gyrase were amplified by PCR, and the...
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