Article
Use of a transient assay for studying the genetic determinants of Fv1 restriction.
Journal of virology - 1 Aug 2000
Bock M, Bishop K N, Towers G, Stoye J P
Abstract excerpt
To probe the genetic determinants controlling the interaction between the retroviral restriction gene Fv1 and its murine leukemia virus target, we set out to develop rapid, transient assays for Fv1 function. Cells were transfected or transduced with Fv1 expression plasmids which can produce green fluorescent protein via an internal ribosome entry site positioned between the Fv1 and green fluorescent protein...
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