T-Cell Clonality Determination Using Polymerase ChainReaction (PCR) Amplification of the T-Cell Receptorgamma-Chain Gene and Capillary Electrophoresis ofFluorescently Labeled PCR Products

Abstract excerpt

We compared the effectiveness of polymerase chain reaction (PCR) and DNA blot analysis (DBA) for detecting clonal T-cell populations and investigated whether a nonradioactive PCR method could be used in routine clinical diagnosis. We analyzed DNA from 117 cases for T-cell clonality by PCR amplification. DBA was performed on 77 of these cases. Denaturing polyacrylamide gel electrophoresis (PCR-PAGE) of...

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