Article
Identification of monoclonal B-cell populations by rapid cycle polymerase chain reaction. A practical screening method for the detection of immunoglobulin gene rearrangements.
The American journal of pathology - 1 Dec 1992
Segal G H, Wittwer C T, Fishleder A J, Stoler M H, Tubbs R R, Kjeldsberg C R
Abstract excerpt
Alternatives to Southern blot hybridization for gene rearrangement analysis are being studied because of the time, labor, cost, and radioisotopes required for this technique. We have utilized a rapid, hot air, thermocycling polymerase chain reaction (PCR) system to examine various lymphoprolifera...
Topics
- B-Lymphocytes
- Base Sequence
- Blotting, Southern
- DNA
- Gene Rearrangement, B-Lymphocyte, Heavy Chain
- Genotype
- Humans
- Immunoglobulin Heavy Chains
- Immunoglobulin Joining Region
- Immunoglobulin Variable Region
- Immunophenotyping
- Molecular Sequence Data
- Polymerase Chain Reaction