Article
Lesions in two Escherichia coli type 1 pilus genes alter pilus number and length without affecting receptor binding.
Journal of bacteriology - 1 Sept 1992
Russell P W, Orndorff P E
Abstract excerpt
We describe the characterization of two genes, fimF and fimG (also called pilD), that encode two minor components of type 1 pili in Escherichia coli. Defined, in-frame deletion mutations were generated in vitro in each of these two genes. A double mutation that had deletions identical to both single lesions was also constructed. Examination of minicell transcription and translation products of parental and mutant...
Topics
- Alleles
- Bacterial Adhesion
- Bacterial Proteins
- Blotting, Southern
- DNA, Recombinant
- Endopeptidases
- Escherichia coli
- Fimbriae, Bacterial
- Genetic Complementation Test
- Hemagglutination Tests
- Mutagenesis