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Article

Mechanical fractionation reveals structural requirements for enteropathogenic Escherichia coli Tir insertion into host membranes.

Infection and immunity - 1 Jul 2000

Gauthier A, de Grado M, Finlay B B

Abstract excerpt

Enteropathogenic Escherichia coli (EPEC) inserts its receptor for intimate adherence (Tir) into host cell membranes by using a type III secretion system. Detergents are frequently used to fractionate infected host cells to investigate bacterial protein delivery into mammalian cells. In this study, we found that the Triton X-100-soluble membrane fraction from EPEC-infected HeLa cells was contaminated with...

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Identifiers

PMID: 10858257
DOI: 10.1128/IAI.68.7.4344-4348.2000
PMCID: PMC101765

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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