Article
Assembly and functional organization of the nucleolus: ultrastructural analysis of Saccharomyces cerevisiae mutants.
Molecular biology of the cell - 1 Jun 2000
Trumtel S, Léger-Silvestre I, Gleizes P E, Teulières F, Gas N
Abstract excerpt
Using Saccharomyces cerevisiae strains with genetically modified nucleoli, we show here that changing parameters as critical as the tandem organization of the ribosomal genes and the polymerase transcribing rDNA, although profoundly modifying the position and the shape of the nucleolus, only partially alter its functional subcompartmentation. High-resolution morphology achieved by cryofixation, together with...
Topics
- Bacterial Proteins
- Binding Sites
- Cell Nucleolus
- DNA, Fungal
- DNA, Ribosomal
- Fungal Proteins
- Mutation
- Nuclear Proteins
- Nucleolus Organizer Region
- Plasmids
- RNA Processing, Post-Transcriptional
- RNA, Fungal
- RNA, Ribosomal